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| Mahé, Kelig; Gourtay, Clemence; Bled--defruit, Geoffrey; Chantre, Celina; De Pontual, Helene; Amara, Rachid; Claireaux, Guy; Audet, C.; Zambonino-infante, Jose-luis; Ernande, Bruno. |
| Otolith shape is an efficient tool for fish stock discrimination. Therefore, it is crucial to identify the environmental parameters that could influence otolith morphogenesis and growth. Current global ocean warming negatively affects the production of polyunsaturated fatty acids (EPA and DHA) by phytoplankton and thus their availability in marine food webs. As EPA and DHA are essential nutrients for most fish species, their deficiency could affect many aspects of fish physiology, notably otolith morphogenesis. The goal of this study was to assess experimentally the combined influences of temperature (T) and dietary polyunsaturated fatty acid (PUFA) content on European seabass (Dicentrarchus labrax) otolith morphogenesis during early life stages. 300... |
| Tipo: Text |
Palavras-chave: N-3 polyunsaturated fatty acids; Otolith shape; Temperature effect; Elliptic Fourier descriptors; Multivariate mixed-effects models; Directional asymmetry. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00588/70054/68017.pdf |
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| Suquet, Marc; Malo, Florent; Gourtay, Clemence; Ratiskol, Dominique; Riobe, Marion; Le Grand, Jacqueline; Queau, Isabelle; Mingant, Christian. |
| A basic cryopreservation protocol for great scallop sperm was recently published, with which thawed sperm survival remained limited to 25% of control values (Suquet et al. 2014). We designed here a set of five experiments to test ways of improving thawed sperm survival and storage by studying the effects of (1) cryoprotectant concentration; (2) sugar incorporation in the extender; (3) egg yolk addition to the extender; (4) sperm dilution rate; and (5) storage capacity of thawed sperm at 4 degrees C. A sixth experiment was then performed to compare rearing performances of larvae produced using thawed sperm from the optimal protocol defined in the present study with those produced using fresh sperm. During the first experiment, a significantly higher... |
| Tipo: Text |
Palavras-chave: Pecten maximus; Mollusc; Sperm; Cryopreservation; Larval rearing. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00359/47033/46946.pdf |
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| Suquet, Marc; Gourtay, Clemence; Donval, Anne; Le Goic, Nelly; Quere, Claudie; Malo, Florent; Le Grand, Jacqueline; Ratiskol, Dominique; Mingant, Christian; Fauvel, Christian. |
| Most publications devoted to the cryopreservation of mollusc sperm have focused on the definition of technical protocols, avoiding the description of sperm quality after thawing. The present study investigated the effects of cryopreservation on sperm quality in the great scallop. Wild scallop were fished during the natural spawning period and conditioned in the hatchery before use. Sperm samples were obtained after intragonadal injection of serotonin and cryopreserved using a previously published protocol. Sperm quality was assessed using a panel of four parameters: sperm motility characteristics, using a a computer assisted sperm analysis plugin with Image J, intracellular ATP content using an ATP-Lite kit, sperm integrity, using flow cytometry and sperm... |
| Tipo: Text |
Palavras-chave: Pecten maximus; Reproduction; Gamete; Sperm; Cryopreservation. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00317/42862/43912.pdf |
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| Gourtay, Clemence; Chabot, Denis; Audet, Celine; Le Delliou, Herve; Quazuguel, Patrick; Claireaux, Guy; Zambonino-infante, Jose-luis. |
| Global climate changes have led to a depletion in omega-3 polyunsaturated fatty acids (n-3 PUFA) in marine phytoplankton that—with food web transfers—could negatively impact fish performance. The aim of this study was to assess the effect of a reduction in the dietary availability of n-3 PUFA on growth performance, organ allometry, and fatty acid composition in juvenile European sea bass (Dicentrarchus labrax) raised at two different temperatures: 15 °C (natural conditions) and 20 °C (global warming scenario). Fish were fed for 5 months with two isoenergetic and isoproteic diets: a reference diet (RD; 1.65% n-3 PUFA on a dry matter basis, DM) used as a proxy of trophic networks where n-3 PUFA were plentiful, and a lower n-3 PUFA diet (LD; 0.73% n-3 PUFA on... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00454/56543/58335.pdf |
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